Denaturation mapping of R factor deoxyribonucleic acid
نویسندگان
چکیده
منابع مشابه
Deoxyribonucleic acid-deoxyribonucleic acid hybridization of R factors.
A new method of estimating deoxyribonucleic acid homology is presented and used to measure the homology between bacterial plasmids of different compatibility groups. Deoxyribonucleic acid of the P-group plasmid RP1, originally isolated in Pseudomonas aeruginosa, has considerable (50%) homology with an N-group plasmid of the Enterobacteriaceae.
متن کاملComposite circular forms of R factor deoxyribonucleic acid molecules.
Two R factors, one (R15) conferring resistance to streptomycin and sulfonamide (SM(r)SU(r)) and the other (222/R3) to streptomycin, sulfonamide, and chloramphenicol (SM(r)SU(r)CM(r)), were transferred to a Proteus mirabilis strain, and deoxyribonucleic acid (DNA) extracted from these strains was subjected to density-gradient centrifugation. R15-DNA formed a single satellite band at a density of...
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The structure of circular pea chloroplast DNA (ctDNA) has been analyzed by denaturation mapping. All of the pea ctDNA molecules that were examined had identical gross base sequences. Denaturation maps were constructed at denaturation levels of 2.5%, 22%, and 44%. These denaturation maps showed that the circular pea ctDNA contained six small AT-rich regions on one-half of the DNA molecule, and t...
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Moles per cent guanine plus cytosine content of 16 lactobacilli provided three taxonomic groups: I, 32.4 to 38.3% with five species; II, 42.7 to 48.0% with six species; III, 49.0 to 51.9% with five species.
متن کاملAlkali denaturation of covalently closed circular duplex deoxyribonucleic acid.
The alkaline pH range over which the double stranded circular DNA of the replicative form of the bacterial virus, 4x174, is reversibly denatured has been determined in order to specify accurately the conditions for a preparative procedure for this DNA based on selective alkali denaturation. The denaturation remains entirely reversible to pH 12.6 at an ionic strength of about 0.25 over a range i...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1975
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.123.3.1035-1042.1975